The protease enzyme production test to bacterial isolate was conducted using Skim Milk Agar. GACTT-3 primers' followed by sequencing process. Molecular identification process was carried out through sequential analysis of 16S rRNA using PCR method using primers forward F: 5'-AGAGTTGATCCTGGCTCAG-3 'and reverse R: 5'- GGTTACCTTGTTAC. The protease enzyme income test was carried out using Skim Milk Agar media. Of the six bacterial isolates isolated from the oncom sample after 120 hours of fermentation, there was one isolate that had protease activity, namely IROD 5. Bacterial isolation and purification was carried out using Nutrient Agar media with spread technique. The purpose of this study was to determine the presence of protease – producing bacteria found on 120-h post - fermented oncom and to identify the bacteria based on its 16S rRNA gene analysis. Among enzymes playing an important role in human life is protease.
Enzymes are complex protein moleculer produced by living cells playing role as catalysts in various chemical processes in the body.
